The microphysical characteristics of a supercooled cloud and the physical response of cloud seeding were analyzed using high-performance rain enhancement aircraft, ground-based S-band dual polarization radar and satellite remote sensing observations in central and southern Hebei Province on January 20, 2022. The results showed that, under the influence of the combination of southwest warm and humid air flow and easterly reflux, a large range of stratiform clouds formed with a cloud base of 1400 m and a cloud top of 2100 m, which were mainly composed of supercooled cloud droplets in the central and southern Hebei Province. Radar observation showed that after cloud seeding at a height of 2100 m, the supercooled cloud droplets rapidly froze into ice crystals, snow and a few graupel particles affected by AgI nucleation. The increase of particle size resulted in an enhanced radar reflectivity echoes in cloud-seeding regions. FY-4A satellite observations showed clear icing cloud tracks occurred after 17～19 min of cloud seeding, which can sustain for about 55 min. After cloud seeding, the supercooled cloud droplets formed ice crystal particles, which gradually grew and fell, causing the cloud top to sink and forming a cloud groove in the cloud top. Compared with the regions without seeding, the reflectance at 0.65 μm and the blackbody brightness temperature at 10.8 μm increased, the reflectance at 3.72 μm decreased within the cloud tracks.