It is still controversial how to quantify the contamination of temperature records by urbanization effects on regional scale, especially in regions where urban built-up expands rapidly like China. Based on historical remote sensing data, this study calculates the urban land-use expansion rate at around 93 meteorological stations in the Yangtze River Delta and classifies these stations into three categories. Using air temperature records from basic/reference meteorological stations, annual and seasonal variations of mean, minimum, and maximum temperature characteristics at different types of stations during the periods from 1980 to 2009 were calculated, and further examine urbanization effects and their contributions to temperature changes at stations that have experienced rapid and moderate urbanization. The results show that almost all the 93 meteorological stations have experienced urban expansion with the expansion rate reaching 1% a^{-1 in} the last three decades. Meanwhile, significant increases in annual and seasonal means of minimum and maximum temperature can be detected at different types of stations. In those stations that experienced rapid urbanization, contributions of urbanization effects to annual mean, minimum, and maximum temperature changes reach up to 35.06%, 34.67%, and 18.42%, respectively. Meanwhile, contributions of urbanization effects at moderate urbanization stations account for 19.35%, 22.22%, and 3.13% of the annual mean, minimum, and maximum temperature changes, respectively. The impact of urbanization on the warming trend varies in different seasons, and the minimum warming trends is found in the winter.